About Léa
French
Native or bilingual
English
Fluent
Spanish
Conversational
Dutch
Basic
Experience
- CNRSAssistant Engineer in Protein BiochemistryRESEARCHFebruary 2016 - January 2021 (4 years and 11 months)Montpellier, FranceMember of the "Structure and functions of biomolecules by NMR" team led by Christian Roumestand and Nathalie Declerck at the Center for Structural Biochemistry in Montpellier (CNRS).Responsible for the expression, purification, and biophysical characterization of proteins for the team.Molecular biology: cloning, sequencing analysis.Microbiology: protein expression in prokaryotic and eukaryotic systems, isotopic labeling for NMR and crystallography.Cell biology: baculovirus production using Sf9 cells, transfection, work in an L2 level laboratory.Crystallography: screening of sparse matrices, refinement, and seeding using the Mosquito liquid handling robot. Use of the Formulatrix Rock Imager robot.Biophysical characterization: SEC-MALS, CD, TSA, and ITC.Supervision of interns (bachelor's and master's).Laboratory management: orders and inventory management.
- EMBLCrystallography Platform EngineerBIOTECHFebruary 2021 - February 2022 (1 year)Grenoble, FranceMember of the "High-Throughput X-ray Crystallography" laboratory- Operator on the high-throughput crystallization platform- Management of fragment screening campaign projects- Research project management- CrystalDirect technology enabling fully automated crystal mounting and processing- Optimization of crystallization conditions- Data collection and analysisPlatform management: orders, inventory management
- CNRSBiochemist EngineerRESEARCHMay 2023 - Today (3 years and 1 month)Paris, FranceBiochemistry engineer at CNRS, I am developing my own research project on exploring the activation mechanism of the γ-TuRC complex (γ-tubulin ring complex) in Drosophila melanogaster, while providing technical and scientific support to my team's projects.I also oversee the operational management of the laboratory as a lab manager (orders, stocks, organization).Specializing in protein biochemistry, I have in-depth expertise in the expression and purification of recombinant proteins, as well as in molecular and structural biology.As head of the protein purification platform at the Institut Jacques Monod, I provide my expertise to other teams within the institute to help them carry out their projects.My skills range from conducting fundamental research projects to optimizing experimental processes, and I regularly contribute to scientific dissemination through presentations and posters at international conferences.
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Education
- PhD in Cell and Molecular BiologyUniversité de Paris Cité2025
- Master's in Structural and Functional BiologyUniversité Claude Bernard Lyon 12023