Freelancer profile translated to English.
Back to original languageAbout Laureen
Immunology study engineer with 13 years of experience and a Master's degree, I work for a company that develops new immunotherapies. My expertise focuses on the development of cell-based assays and flow cytometry, as well as FlowJo/Prism analysis and the writing of protocols/SOPs/reports.
French
Native or bilingual
English
Conversational
Remote only
Primarily works remotely
Experience
- EnteromeAssistant EngineerPHARMACEUTICALS INDUSTRYFebruary 2019 - December 2024 (5 years and 10 months)Évry, FranceEvaluation of the immunomodulatory activity of proteins and peptides, characterization and study of the mechanism of action of molecules of interest.• Primary cell culture (PBMC isolation by Ficoll, magnetic sorting) and cell line maintenance• Development and optimization of cytokine secretion detection systems (HTRF, ELISA, alphaLISA, LegendPlex) or reporter systems• Development of cellular models: mo-DCs, TH2, MDSC, MLR, M2• Phenotyping and binding assays by flow cytometry• Gene expression study by qPCR• Protein production and purification: in prokaryotic or cell-free systems, purification by affinity and QC by SDS PAGE and Western blot• Management, monitoring, and production of a protein bank in a cell-free system, HTS
- Blue Bees therapeuticsStudy EngineerBIOTECHJanuary 2025 - Today (1 year and 5 months)Paris, FranceDevelopment of immunotherapies in immuno-oncology. Characterization and study of a compound's mechanism of action for clinical development.Evaluation of the immune modulation induced by the compound on immune cells from healthy donors and patients using multiparametric immunophenotyping (flow cytometry; target populations: neutrophils, MDSCs, DCs, monocytes, T cells, NK cells, B cells).Study of compound binding to immune cells (PBMCs or whole blood).Pharmacodynamic analysis in murine tumor models (immunophenotyping).Optimization of 14-color flow cytometry panels.Participation in project monitoring and experimental organization
- H-IMMUNE/HIFIBIO TherapeuticsSenior Research TechnicianMay 2017 - February 2019 (1 year and 9 months)Development of a method for producing fully human antibodies by in vitro immunization and in vitro antibody characterization.• PBMC isolation by Ficoll, magnetic sorting, and B lymphocyte characterization by flow cytometry• Participation in single cell cloning experiments (PCR, cloning, sequencing, clone selection)• Production and purification of antibodies and recombinant proteins (E.coli, HEK, CHO)• Antibody validation by ELISA• Evaluation of the activity of several antibodies on T cells (Treg, apoptosis) by flow cytometry
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Education
- Master of Science in Medicines and Health ProductsUniversité Paris Saclay2024Master Science du Médicament et des produits de santé
- Professional LicenseESTBA and Université Paris 72009Licence Professionnelle
Skill set
Categories
- Other